September
Back to the articles list |
Back to browse issues page
1- School of Medicine, Iran University of Medical Sciences, Tehran, Iran
2- Lois Pope Life Center, University of Miami, Miami, FL, USA
3- Physiology Research Center, Iran University of Medical Sciences, Tehran, Iran & Center of experimental and comparative study, Iran University of Medical Sciences, Tehran, Iran ,nasirinezhad.f@iums.ac.ir
2- Lois Pope Life Center, University of Miami, Miami, FL, USA
3- Physiology Research Center, Iran University of Medical Sciences, Tehran, Iran & Center of experimental and comparative study, Iran University of Medical Sciences, Tehran, Iran ,
Abstract: (15 Views)
Background and Objective: Neuropathic pain, characterized as a persistent ailment, presents considerable therapeutic obstacles owing to the restricted effectiveness of contemporary interventions. This study aimed to assess the therapeutic efficacy of conditioned medium (CM) derived from human amniotic membrane mesenchymal stem cells (MSCs) in mitigating neuropathic pain and regulating spinal calcitonin gene-related peptide (CGRP) concentrations in a chronic constriction injury (CCI) model. CGRP, a neuropeptide released by primary sensory neurons, plays a central role in pain transmission, neurogenic inflammation, and central sensitization in neuropathic conditions. Measuring CGRP was chosen to evaluate CM's efficacy because its modulation reflects alterations in nociceptive signaling pathways, providing mechanistic insights into pain relief beyond behavioral outcomes.
Methods: Adult male Sprague-Dawley rats underwent CCI to induce neuropathic pain, followed by intrapreneurial administration of CM or vehicle above the ligature site. Mechanical and cold allodynia were assessed using the Randall-Selitto and acetone tests at baseline and up to day 14 post-intervention. Spinal cord segments (L4-L6) were harvested at study termination for Western blot analysis of CGRP levels, with statistical significance set at P < 0.05.
Results: CM-treated rats showed significant reductions in mechanical and cold allodynia by day 7, sustained through day 14, compared to vehicle and CCI-only groups (P < 0.05). Spinal CGRP levels were markedly lower in the CM group than in the control group (P < 0.001).
Conclusion: These findings suggest that amniotic MSC-derived CM mitigates neuropathic pain by modulating CGRP-mediated pain signaling and altering nociceptive thresholds. This study highlights CM as a promising non-cellular therapeutic for neuropathic pain, warranting further mechanistic and preclinical studies to explore its clinical potential.
Methods: Adult male Sprague-Dawley rats underwent CCI to induce neuropathic pain, followed by intrapreneurial administration of CM or vehicle above the ligature site. Mechanical and cold allodynia were assessed using the Randall-Selitto and acetone tests at baseline and up to day 14 post-intervention. Spinal cord segments (L4-L6) were harvested at study termination for Western blot analysis of CGRP levels, with statistical significance set at P < 0.05.
Results: CM-treated rats showed significant reductions in mechanical and cold allodynia by day 7, sustained through day 14, compared to vehicle and CCI-only groups (P < 0.05). Spinal CGRP levels were markedly lower in the CM group than in the control group (P < 0.001).
Conclusion: These findings suggest that amniotic MSC-derived CM mitigates neuropathic pain by modulating CGRP-mediated pain signaling and altering nociceptive thresholds. This study highlights CM as a promising non-cellular therapeutic for neuropathic pain, warranting further mechanistic and preclinical studies to explore its clinical potential.
Keywords: Calcitonin gene-related peptide (CGRP) levels, Chronic constriction injury (CCI), Conditioned medium (CM), Human amniotic stem cells, Neuropathic pain
Send email to the article author
| Rights and permissions | |
 |
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License. |
Articles Copyright © The Author(s).
Owned by Hamadan University of Medical Sciences.
Published by UMSHA Press
Journal Tel: +989025126654
Publisher Tel: +985136014377
Website: http://ajnpp.umsha.ac.ir
Email: avicennajnpp[at]gmail.com
