Volume 11, Issue 4 (November 2024)
Avicenna J Neuro Psycho Physiology 2024, 11(4): 0-0 |
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Afraz S, Karami M. Blockade of dopamine type 2 receptors by sulpiride, intra VMH, ameliorates the fertility-disrupting effects of morphine in female Wisar rats. Avicenna J Neuro Psycho Physiology 2024; 11 (4)
URL: http://ajnpp.umsha.ac.ir/article-1-512-en.html
URL: http://ajnpp.umsha.ac.ir/article-1-512-en.html
1- MSc student, Dept. of Biology, Faculty of Basic Sciences, Shahed University, Tehran, Iran
2- Associate Prof, Dept. of Biology, Faculty of Basic Sciences, Shahed University, Tehran, Iran ,karami@shahed.ac.ir
2- Associate Prof, Dept. of Biology, Faculty of Basic Sciences, Shahed University, Tehran, Iran ,
Abstract: (115 Views)
Introduction and Objective: Polycystic ovary syndrome (PCOS) is a reproductive disorder that causes infertility. Morphine, when injected into the ventromedial hypothalamus (VMH), is effective in inducing PCO, but the mechanism of this effect, particularly concerning dopamine (DA), remains unclear. The study aims to understand how DA and its receptors contribute to this disruption.
Materials and Methods: Virgin animals weighing 220-250 g and aged 8-9 weeks were randomly divided into control, morphine alone, sulpiride alone, and sulpiride + morphine groups that received sulpiride before an effective dose of morphine. Morphine (0.1-0.4 μg/rat) was injected into the VMH (AP coordinates: -1.92; ventral: 9 mm; lateral: 0.5 mm). Sulpiride (0.1-0.4 μg/rat) was injected, intra-VMH, alone or before morphine (0.4 μg/rat, intra-VMH). The control group received saline merely (1 μL/rat, intra-VMH). The animals were anesthetized three days later, and after surgery, the ovaries, uterus, and brain were collected and examined in 10% formalin. All data were statistically analyzed.
Results: Ovaries of rats treated with morphine and not in the sulpiride single groups showed PCO features compared to the control group, but the number of cysts was significantly reduced in the sulpiride + morphine samples. Morphine and sulpiride did not significantly induce uterine inflammation. Also, no significant effect on VMH neurons was observed in any group.
Conclusion: These results indicate that morphine disrupts follicular growth and that this effect is partially reversed by blocking DA type 2 receptor signaling.
Materials and Methods: Virgin animals weighing 220-250 g and aged 8-9 weeks were randomly divided into control, morphine alone, sulpiride alone, and sulpiride + morphine groups that received sulpiride before an effective dose of morphine. Morphine (0.1-0.4 μg/rat) was injected into the VMH (AP coordinates: -1.92; ventral: 9 mm; lateral: 0.5 mm). Sulpiride (0.1-0.4 μg/rat) was injected, intra-VMH, alone or before morphine (0.4 μg/rat, intra-VMH). The control group received saline merely (1 μL/rat, intra-VMH). The animals were anesthetized three days later, and after surgery, the ovaries, uterus, and brain were collected and examined in 10% formalin. All data were statistically analyzed.
Results: Ovaries of rats treated with morphine and not in the sulpiride single groups showed PCO features compared to the control group, but the number of cysts was significantly reduced in the sulpiride + morphine samples. Morphine and sulpiride did not significantly induce uterine inflammation. Also, no significant effect on VMH neurons was observed in any group.
Conclusion: These results indicate that morphine disrupts follicular growth and that this effect is partially reversed by blocking DA type 2 receptor signaling.
Article Type: Research Article |
Subject:
Substance abuse, dependence, addiction
Received: 2025/02/2 | Accepted: 2025/05/31 | Published: 2024/11/5
Received: 2025/02/2 | Accepted: 2025/05/31 | Published: 2024/11/5
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