en تخصصي Special پژوهشي Research Article <aside style="box-sizing: border-box; font-family: Lato, Arial; word-wrap: break-word; margin-bottom: 10px; font-size: 11px;"> <p style="box-sizing: border-box; word-wrap: break-word; margin: 0px 0px 10px; font-size: 12px; text-align: justify;"><span style="box-sizing: border-box; word-wrap: break-word; font-weight: 700;">Background:</span>&nbsp;Side effects of synthetic analgesic drugs in the clinical practice have drawn researchers&rsquo; attention on developing the herbal medicine as more appropriate analgesic agents.</p> </aside> <aside style="box-sizing: border-box; font-family: Lato, Arial; word-wrap: break-word; margin-bottom: 10px; font-size: 11px;"> <p style="box-sizing: border-box; word-wrap: break-word; margin: 0px 0px 10px; font-size: 12px; text-align: justify;"><span style="box-sizing: border-box; word-wrap: break-word; font-weight: 700;">Objectives:</span>&nbsp;This study aimed to investigate the antinociceptive effect of hydroalcoholic leaf extract of&nbsp;<i style="box-sizing: border-box; word-wrap: break-word;">Bryonia dioica</i>&nbsp;(HEBD) on male rats.</p> </aside> <aside style="box-sizing: border-box; font-family: Lato, Arial; word-wrap: break-word; margin-bottom: 10px; font-size: 11px;"> <p style="box-sizing: border-box; word-wrap: break-word; margin: 0px 0px 10px; font-size: 12px; text-align: justify;"><span style="box-sizing: border-box; word-wrap: break-word; font-weight: 700;">Materials and Methods:</span>&nbsp;In this experimental study, 42 adult male rats were divided into 7 groups: control, HEBD (80, 100, and 300 mg/kg, ip), morphine (1 mg/kg, ip), indomethacin (1 mg/kg, ip), and naloxone (1 mg/kg ip). In order to assess the analgesic effects of the extract, writhing, tail-flick, and formalin tests were used. Also, Tukey post hoc and 1-way analysis of variance (ANOVA) tests were used to analyze the data.</p> </aside> <aside style="box-sizing: border-box; font-family: Lato, Arial; word-wrap: break-word; margin-bottom: 10px; font-size: 11px;"> <p style="box-sizing: border-box; word-wrap: break-word; margin: 0px 0px 10px; font-size: 12px; text-align: justify;"><span style="box-sizing: border-box; word-wrap: break-word; font-weight: 700;">Results:</span>&nbsp;Using writhing, tail-flick, and formalin tests, dramatic antinociceptive effects were observed for HEBD at the dose of 300 mg/kg with P < 0.01. The results of data analysis also revealed that there was no significant difference between indomethacin and dose of 300 mg/kg of extract in formalin test. LD50 of the plant was 4200 mg/kg.</p> </aside> <aside style="box-sizing: border-box; font-family: Lato, Arial; word-wrap: break-word; margin-bottom: 10px; font-size: 11px;"> <p style="box-sizing: border-box; word-wrap: break-word; margin: 0px 0px 10px; font-size: 12px; text-align: justify;"><span style="box-sizing: border-box; word-wrap: break-word; font-weight: 700;">Conclusions:</span>&nbsp;The experimental data verified that HEBD leaves displayed remarkable antinociceptive activity. Furthermore, the nonselective opioid receptor antagonist, naloxone blocked the antinociceptive effect of the extract in all tests, suggesting that HEBD may act both at peripheral and central levels.</p> </aside> Pain, Plants, Medicinal, Bryonia, Formaldehyde, Rats 13 17 http://ajnpp.umsha.ac.ir/browse.php?a_code=A-10-2-20&slc_lang=en&sid=1 Mohammad Zarei 100319475328460082 100319475328460082 No Neurophysiology Research Center, Hamadan University of Medical Sciences, Hamadan, IR Iran ; Department of Physiology, Hamadan University of Medical Sciences, Hamadan, IR Iran Saeed Mohammadi smiauhphd.sm@gmail.com 100319475328460083 100319475328460083 No Department of Biology, Faculty of Basic Sciences, Islamic Azad University, Hamadan, IR Iran Nasreen Abolhassani 100319475328460084 100319475328460084 Yes Department of Biology, Faculty of Basic Sciences, Science and Research Branch, Islamic Azad University, Tehran, IR Iran Mahtab Asgari Nematian 100319475328460085 100319475328460085 No Department of Biology, Hamadan Branch, Payam-noor University, Hamadan, IR Iran