en تخصصي Special پژوهشي Research Article <aside style="box-sizing: border-box; font-family: Lato, Arial; word-wrap: break-word; margin-bottom: 10px; font-size: 11px;"> <p style="box-sizing: border-box; word-wrap: break-word; margin: 0px 0px 10px; font-size: 12px; text-align: justify;"><span style="box-sizing: border-box; word-wrap: break-word; font-weight: 700;">Background:</span>&nbsp;The elevated plus-maze (EPM) has been broadly used to investigate anxiolytic and anxiogenic compounds. There is little information about the effect of interaction between calcium channels and cannabinoid system on the phenomenon of anxiety.</p> </aside> <aside style="box-sizing: border-box; font-family: Lato, Arial; word-wrap: break-word; margin-bottom: 10px; font-size: 11px;"> <p style="box-sizing: border-box; word-wrap: break-word; margin: 0px 0px 10px; font-size: 12px; text-align: justify;"><span style="box-sizing: border-box; word-wrap: break-word; font-weight: 700;">Objectives:</span>&nbsp;This study aimed to examine the effects of acute and chronic coadministration of AM251, as cannabinoid CB1 receptor antagonist, and Verapamil, as L-type Ca<span style="box-sizing: border-box; word-wrap: break-word; position: relative; font-size: 9px; line-height: 0; vertical-align: baseline; top: -0.5em;">2+</span>&nbsp;channels blocker, on EPM test in rats.</p> </aside> <aside style="box-sizing: border-box; font-family: Lato, Arial; word-wrap: break-word; margin-bottom: 10px; font-size: 11px;"> <p style="box-sizing: border-box; word-wrap: break-word; margin: 0px 0px 10px; font-size: 12px; text-align: justify;"><span style="box-sizing: border-box; word-wrap: break-word; font-weight: 700;">Materials and Methods:</span>&nbsp;The data were obtained from male Wistar rat, weighing 220 to 260 g. Animals were allocated to five groups: Control, Verapamil, AM251, acute Verapamil + AM251, and chronic (injection for 8 days) Verapamil + AM251 groups. The percentage of entries into the open arms of the EPM, the time spent in the open arms, and the number of entries into the closed arms during ten minutes was recorded.</p> </aside> <aside style="box-sizing: border-box; font-family: Lato, Arial; word-wrap: break-word; margin-bottom: 10px; font-size: 11px;"> <p style="box-sizing: border-box; word-wrap: break-word; margin: 0px 0px 10px; font-size: 12px; text-align: justify;"><span style="box-sizing: border-box; word-wrap: break-word; font-weight: 700;">Results:</span>&nbsp;Intraperitoneally (IP) injection of AM251 before EPM trial decreased open arms exploration and open arm entry. On the other hand, Verapamil increased open arms exploration and open arm entry. Combined injection of Verapamil and AM251 had conflicting effects on the responses of each of these two compounds alone. AM251 and Verapamil had no effects on the number of closed arm entries.</p> </aside> <aside style="box-sizing: border-box; font-family: Lato, Arial; word-wrap: break-word; margin-bottom: 10px; font-size: 11px;"> <p style="box-sizing: border-box; word-wrap: break-word; margin: 0px 0px 10px; font-size: 12px; text-align: justify;"><span style="box-sizing: border-box; word-wrap: break-word; font-weight: 700;">Conclusions:</span>&nbsp;IP injection of CB1 receptor antagonist might have an anxiogenic profile in rat, whereas calcium channel blocker attenuated the anxiogenic effect of AM251. Our results suggest that there is an interaction between functions of L-type Ca<span style="box-sizing: border-box; word-wrap: break-word; position: relative; font-size: 9px; line-height: 0; vertical-align: baseline; top: -0.5em;">2+</span>channels and cannabinoid system in anxiety.</p> </aside> Verapamil, L-Type Calcium Channels, AM251, Rat, Anxiety 62 68 http://ajnpp.umsha.ac.ir/browse.php?a_code=A-10-2-15&slc_lang=en&sid=1 Alireza Komaki alirezakomaki@gmail.com 100319475328460059 100319475328460059 Yes Neurophysiology Research Center, Hamadan University of Medical Sciences, Hamadan, IR Iran Aezam Haghgooyan 100319475328460060 100319475328460060 No Neurophysiology Research Center, Hamadan University of Medical Sciences, Hamadan, IR Iran Siamak Shahidi 100319475328460061 100319475328460061 No Neurophysiology Research Center, Hamadan University of Medical Sciences, Hamadan, IR Iran Abdolrahman Sarihi 100319475328460062 100319475328460062 No Neurophysiology Research Center, Hamadan University of Medical Sciences, Hamadan, IR Iran Iraj Saleh 100319475328460063 100319475328460063 No Neurophysiology Research Center, Hamadan University of Medical Sciences, Hamadan, IR Iran