Volume 8, Issue 2 (May 2021)
Avicenna J Neuro Psycho Physiology 2021, 8(2): 64-70 |
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Jabbari S, Bananej M, Zarei M, Komaki A, Hajikhani R. Possible Involvement of Serotonergic Mechanism(s) in the Antinociceptive Effects of kaempferol. Avicenna J Neuro Psycho Physiology 2021; 8 (2) :64-70
URL: http://ajnpp.umsha.ac.ir/article-1-285-en.html
URL: http://ajnpp.umsha.ac.ir/article-1-285-en.html
1- Department of Biology, Faculty of Life Sciences, Islamic Azad University, Tehran North Branch, Tehran, Iran.
2- Department of Biology, Faculty of Life Sciences, Islamic Azad University, Tehran North Branch, Tehran, Iran , Maryam.bananej23@gmail.com
3- Department of Physology, Scihool of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
4- Neurophysiology Research Center, Hamadan University of Medical Sciences, Hamadan, Iran.
5- Department of Biology, Faculty of Life Sciences, Islamic Azad University, Tehran North Branch, Tehran, Iran
2- Department of Biology, Faculty of Life Sciences, Islamic Azad University, Tehran North Branch, Tehran, Iran , Maryam.bananej23@gmail.com
3- Department of Physology, Scihool of Medicine, Hamadan University of Medical Sciences, Hamadan, Iran
4- Neurophysiology Research Center, Hamadan University of Medical Sciences, Hamadan, Iran.
5- Department of Biology, Faculty of Life Sciences, Islamic Azad University, Tehran North Branch, Tehran, Iran
Abstract: (1235 Views)
Background and Objectives: A flavonoid kaempferol (KM) exerts an anti-inflammatory effect and is reportedly capable of preventing metabolic diseases. Nonetheless, a limited number of studies have been carried out on the antinociceptive effects of kaempferol.
Objectives: The present study aimed to investigate the involvement of serotonin receptors in the antinociceptive-like activity of KM in male Wistar rats using the tail-flick test.
Materials and Methods: The compounds (i.e., KM, morphine, and diclofenac) were intracerebroventricularly administered to rats for the examination of central effects on the thermal pain using the tail-flick test. For the evaluation of the involvement of serotonin receptors in the possible antinociceptive effects of kaempferol, several antagonists (i.e., tropisetron, ketanserin, GR113808, WAY 100635, and penbutolol) were used. Additionally, locomotor activity and motor responses were investigated by the rotarod test after KM treatment.
Results: The intracerebroventricular microinjections of KM showed antinociceptive effects using the tail-flick test. The pretreatment with tropisetron as a 5-HT3 receptor antagonist at 1 and 10 mg completely reversed the KM-related antinociception. Furthermore, ketanserin (5-HT2A receptor antagonist) and GR113808 (5-HT4 receptor antagonist) both at 10 mg reduced KM-related antinociception; however, 5-HT1A receptor antagonist WAY 100635 and 5-HT1B antagonist penbutolol did not decrease KM-related antinociception. All KM doses were not observed with a significant effect on locomotor activity or motor reactions.
Conclusion: The results of the current study suggested that serotonergic receptors (i.e., 5-HT2A, 5-HT3, and 5-HT4) are effective in the KM antinociceptive activity in male rats.
Objectives: The present study aimed to investigate the involvement of serotonin receptors in the antinociceptive-like activity of KM in male Wistar rats using the tail-flick test.
Materials and Methods: The compounds (i.e., KM, morphine, and diclofenac) were intracerebroventricularly administered to rats for the examination of central effects on the thermal pain using the tail-flick test. For the evaluation of the involvement of serotonin receptors in the possible antinociceptive effects of kaempferol, several antagonists (i.e., tropisetron, ketanserin, GR113808, WAY 100635, and penbutolol) were used. Additionally, locomotor activity and motor responses were investigated by the rotarod test after KM treatment.
Results: The intracerebroventricular microinjections of KM showed antinociceptive effects using the tail-flick test. The pretreatment with tropisetron as a 5-HT3 receptor antagonist at 1 and 10 mg completely reversed the KM-related antinociception. Furthermore, ketanserin (5-HT2A receptor antagonist) and GR113808 (5-HT4 receptor antagonist) both at 10 mg reduced KM-related antinociception; however, 5-HT1A receptor antagonist WAY 100635 and 5-HT1B antagonist penbutolol did not decrease KM-related antinociception. All KM doses were not observed with a significant effect on locomotor activity or motor reactions.
Conclusion: The results of the current study suggested that serotonergic receptors (i.e., 5-HT2A, 5-HT3, and 5-HT4) are effective in the KM antinociceptive activity in male rats.
Article Type: Research Article |
Subject:
Cognition
Received: 2020/07/10 | Accepted: 2020/08/5 | Published: 2021/05/20
Received: 2020/07/10 | Accepted: 2020/08/5 | Published: 2021/05/20
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